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In a recently published review, the advantages of eryptosis over hemolysis as a toxicological endpoint have been summarized, emphasizing that eryptosis in vitro testing has a higher sensitivity, is more accurate and reproducible, provides better quality control, and can shed light on the cytotoxicity-mediating molecular mechanisms compared with hemolysis [450]

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This system combines acidification and aeration to reduce the bicarbonate concentration to a desired maximum value of 0.5 mmol/l