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Since creatinine is a byproduct of muscle metabolism, individuals with greater muscle mass, those who engage in more physical activity, or those generally in better health produce more creatinine than people with lower muscle mass, physical activity levels, and poorer overall health

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Samples were mixed by vortexing and incubated for 10 min 4 C with rotation, before centrifugation (10 min, 1,000 g , 4 C)

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