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The protein concentration was measured by BCA kit (Thermo Scientific TM Pierce TM BCA Protein Assay Kit, Waltham, MA, United States)

When documentation is inconsistent, two runs that appear identical may actually differ in a subtle but important way, such as the lot identifier recorded, the assumed concentration framework used in preparation notes, the timing window applied during staging, or the storage assumptions referenced in a worksheet

In addition, in cultured cells, overexpression or underexpression of GCH1 made cancer cells correspondingly resistant or sensitized to ferroptosis

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