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The observation that different BBL isoforms act to preferentially produce different nicotine enantiomers suggests that BBL enzymes may utilize the achiral DMN molecule as a substrate in the final step of nicotine biosynthesis [19]

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After equilibration for 60 min at the optimal resting tension of 20 mN, maximal contractility was determined with a depolarizing solution containing 60 mM KCl