When cultures reached OD600 of 0
qPCR quantification graphs for detecting the 3´ amplicon and 5´ amplicon of GUSB cDNA produced by Epicentre's MMLV High Performance Reverse Transcriptase Kit and two other commercially available reverse transcriptase enzymes
Reduces DNA cross contamination
16/26 (62%)
and cell pellets were photographed under long-wavelength UV illumination
Plasmid-Safe-ATP-Dependent DNase, 1,000 U @ 10 U/uL DNA When cultures reached OD600 ofRemove contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC preps Digest contaminating linear DNA without digesting nicked or closed circular dsDNA or supercoiled DNA (plasmids, fosmids, etc.) Use as a final purification step for plasmid, cosmid, fosmid, and BAC vector and clone preparations Utilise whenever protecting circular dsDNA is important Plasmid Safe ATP Dependent DNase selectively removes contaminating bacterial