Primary antibodies to DPEP-1 (Proteintech, #12222-1-AP, 1:1000), 4-HNE (Abcam, #ab46545, 1:1000), FTH1 (Abcam, #ab65080, 1:1000), GCLM (Abcam, #EPR6667, 1:1000), GCLC (Abcam, #EP13475, 1:2000), ZBP1 (Adipogen, #AG-20B-0010-c100, 1:1000), FSP1 (Santa Cruz, #sc-377120, 1:5000), and -actin (Cell Signaling, #3700S, 1:500) were diluted in skimmed milk (Carl Roth, #T145.2)
Unlike common dietary supplements in the US, we have prepared thoroughly independent and differentiated products tailored for Koreans
E.GumprichtE.DahlR.GrallaJ.et al (2007)
The Acsl4 conditional knockout (cKO) model ( Acsl4 fl/fl Ksp Cre ) was engineered through crossbreeding of Acsl4 fl/fl mice with Ksp Cre transgenic strains, while the Fsp1 fl/fl Ksp Cre cKO mice were generated using analogous breeding strategies
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