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Additional substitutions of 2´-dNTPs (or 2´-modified-dNTPs) for canonical rNTPs will further reduce yield of the transcript produced
Quality Control: Exo-Minus Klenow DNA Polymerase (D355A
100 mM (NH4)2SO4
the background of non-specific amplification was much lower with EconoTaq (compare “+”and “–“ lanes for EconoTaq and Tfl in Figure 4)
Cell lysates were prepared with or without OmniCleave Endonuclease from E
Duraseal, 4" x 500' PCR RELATED Additional substitutions of 2´-dNTPs (orDuraSeal the durable, heat and solvent resistant stretch film, has substantial advantages over common laboratory stretch films. Its' superior heat resistant feature allows for containers to be incubated in boiling water baths without suffering the characteristic melting of parraffin based alternatives. Since DuraSeal is polyethylene based and free of any adhesives, it resists essentially all caustic agents and organic solvents. DuraSeal is easy to