DNA Ligation size:For 6 groups of 4-5 or 24-30 students. Each kit contains four rulers

Each kit contains four rulers

2 × 105 3

This enzyme has optimal activity at 65°C

Figure 2 (click to enlarge)

Exonuclease III 10X Reaction Buffer: 330 mM Tris-acetate (pH 7

DNA Ligation size:For 6 groups of 4-5 or 24-30 students. Each kit contains four rulersFollowing restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyzes the formation of phosphodiester bonds. The DNA Ligation kit teaches students about ligation as they ligate several DNA fragments together to make larger pieces of DNA that are easily identified by agarose