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The following secondary antibodies were utilized: Alexa Fluor 488 goat anti-rabbit antibody (1:400 for IF), Alexa Fluor 633 goat anti-mouse antibody (1:400 for IF), and horseradish peroxidase (HRP)-conjugated antibody (goat anti-rabbit or goat anti-mouse, 1:10,000 for IB)

Curr Med Chem (2012) 19:639

Chewing tobacco or spitting tobacco is one of the common types of smokeless tobacco

The US Surgeon General recently declared e-cigarette use among youth an epidemic, with the use of e-cigarettes among US high school students nearly doubling from 2017 to 2018 (11.7% to 20.8%)

However, the teams have changed their composition