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Several substances have been identified in e-cigarettes in addition to nicotine, including solvent carriers, tobacco-specific nitrosamines, aldehydes, metals, volatile organic compounds, phenolic compounds, polycyclic aromatic hydrocarbons, flavourings, tobacco alkaloids, and drugs 16

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Cells were plated in 24- or 6-well plates (EM), grown to confluence and placed overnight in BEBM containing transferrin, insulin, gentamicin and amphotericin B (Sigma-Aldrich)