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For the phosphatidylcholine hydroperoxide (PCOOH)/Fe 2+ system, PCOOH was enzymatically synthesized from 1-palmitoyl-2-linoleoyl- sn -glycero-3-phosphocholine (16:0-18:2 PC, Avanti Polar Lipids) using soybean lipoxygenase-1 and chromatographically purified 47