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The coverslips were then washed with PBS and the cells were fixed with 4% PFA for 15 min, washed with 0.05% Tween 20 (Promega, WI, USA) diluted in PBS (PBST), permeabilized with 0.2% Triton X-100 (Promega, WI, USA) for 10 min, and blocked with 3% BSA in PBST for 3 h

Eve was launched in 1971 to compete with Philip Morris corporations Virginia Slims cigarette, as a more conventionally feminine brand of cigarette to target the growing womens market, particularly marketed towards Black women

READ MORE Q&A: A Lighthouse Called Kanata May 08, 2024 With The Treasure House Fair soon opening its doors, we are delighted to showcase some of the wonderful exhibitors who will be joining us this year