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They found that sustained exposure to CC or ENDS caused a severe systemic inflammatory response, with increased activation of the NLRP3 inflammasome in neutrophils and macrophages, and enhanced dendritic cell-dependent activation of Th1 and Th17 cells in the lungs

In extreme situations, though, it may be viable over the short term

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The sample size was based on the principle of data saturation 24 , seeing saturation when new data collected repeats what was expressed in the previously collected data 25