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For analysis of the total nitrogen (TN), total phosphorus (TP), total alkaloids (TA), amylum, protein, pH, petroleum ether extract (PEE), and total free amino acids (TFAA), the samples were pulverized in liquid nitrogen using a mortar and pestle and stored in an ultra-low-temperature freezer at 80C

However, the authors also noted that the tests were conducted at 20C, and that the results may not hold true in a hotter environment (e.g., in a 40C room during an extreme heat event)

the characterization of dual-labeled Cy5-TMV-PEG particles are included in Figure S3

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At that figure, a quarterback has to be the best in the NFL to justify the outlay in a league with a hard salary cap