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In accordance with these results, upregulation of filaggrin was also observed in oral keratinocytes of smokers 19 , in keratinocyte cell cultures 19, 20 or in reconstructed human epidermis 21 exposed to cigarette smoke extract, and porcine skin explants exposed to cigarette smoke (our unpublished data)

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This has implications for allergic risk, as impaired Th1 function in the perinatal period has been associated with allergic risk in many studies [79,80]