Ethnopharmacology 319 (Pt 3), 117345
Quantitative PCR (qPCR) was performed using the QuantStudio 1 system (Applied Biosystems, Foster City, CA, USA), and PCR and data analysis were performed following previously described methods [55, 56]
Subsequently, 5 ml of bacterial cultures ( oxyR-msrA , oxyR-msrB or no plasmid) at an OD 600 of 0.5 were mixed with 5 ml of NFs in a clean microcentrifuge tube and incubated at 37 C, 160 rpm throughout the experiment
It changes the concentration, which changes the draw volume needed to measure the same target amount
Of these, four were excluded before any attempts at pump implantation (phase 1): one before the initial workup died suddenly owing to poor cardiac status, and three were excluded during the initial workup (owing to mild fluctuations, psychosis during the acute l-dopa administration test and benign brain tumor)