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Freebase Nicotine: Freebase nicotine is usually a PG/VG mixture and is the traditional nicotine base in mostly all e-liquids found on the market

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Isocratic separation of the ammonium ion was achieved using a 20mM methanesulphonic acid (MSA) eluent prepared by the EG40 eluent generator (Thermo Scientific, Sunnyvale, CA) that required only a deionized water source