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It snaked out of the Andes from the burning leaves of Nicotiana tabacum some 6,000 years ago, spreading across the lands that would come to be known as South America and the Caribbean, until finally reaching the eastern shores of North America

Polymerase chain reaction (PCR) primers were designed based on National Center for Biotechnology Information (NCBI) reference sequences, and sequencing was performed using the Sanger method

WHO report on the global tobacco epidemic: Country profile, Egypt

[PubMed] 102.Paleari L, Negri E, Catassi A, Cilli M, Servent D, DAngelillo R, et al

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