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It contained several heavy-riffing numbers, with lyrics penned by Butler and yowled convincingly by Osbourne, which helped define the metal sound: War Pigs, Paranoid, Hand of Doom and what ultimately became the groups best-known signature, Iron Man. The original lineup issued its highest-charting release, Master of Reality, in 1971

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coli , apo-ACP is posttranslationally modified and converted to active holo-ACP via covalent attachment of 4-phosphopantetheine (4-PP) from coenzyme A (CoA) by the enzyme holo-ACP synthase (AcpS) (Cronan and Thomas 2009)