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Membranes were washed three times in 1PBS and positive signals were developed with enhanced chemiluminescence kit (Amershan Pharmacia Biotech, Little Chalfont Buckinghamshire, England)

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Highlights GDC temporarily decreases TEER in Caco-2/HT29-MTX monolayers, without cytotoxicity GDC decreases OCLN fluorescence intensity and increases lactulose paracellular transport GDC increases acidic mucins production and decreases intracellular cAMP GDC treated monolayers are biocompatible with Infant Formula digesta 1 Introduction The infant gut barrier has a higher paracellular permeability during early development ( There are ethical and logistical challenges of conducting clinical trials in early infancy to understand the gut barrier

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