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If children live in a home where parents are smokers, there is a likelihood that they are being affected by secondhand smoke

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Troy Balderson (R-OH): Alright, thank you Madam Chair

Western blotting Human peripheral lung tissue Crushed human lung tissue was lysed in buffer containing 50 mM Tris pH 7.4, 150 mM NaCl, 0.1% NP40 and protease inhibitor cocktail (Roche)