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After incubation with blocking buffer (PBS-C/M containing 0.5% BSA, 0.5% Triton X-100, and 0.2 mg/ml sodium azide) for 30 min at room temperature, the membranes were incubated with anti-ZO-1 antibody in PBS-C/M for 1 h at room temperature

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This guide strengthens the case for ZYNs over vapes by looking at the health impacts, ease of use, and cost-effectiveness, among other factors, of both products