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After exposure, cells were fixed with 4% paraformaldehyde for 10 min, then stained with 1x Phalloidin-iFluor 594 ab176757 (Abcam, Cambridge, United Kingdom) for 1 h, following the assay procedure recommended by the manufacturer

The testing was undertaken by core members of the Ipsos MORI research team

Age appears to play a significant factor in vaping

I do understand that this seems unlikely since cigarettes cost money and only a tiny fraction may go to the extent of smoking more for the same of winning a pointless street poll, unlike online polls where participation does not cost anything at all