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Over time, the confluence of these effects can cause damage to the lining itself and even potentially to the DNA of the epithelial cells

Urinary cotinine levels are elevated in infants of smoking mothers

RNA isolation and real-time PCR Total RNA from cells exposed to extracts for 48 h was extracted using TRIzol reagent (Invitrogen) and quantified using a nano-drop followed by Reverse Transcriptase PCR using random primers per manufacturers recommendations

Stephanie Christenson, an assistant professor in the Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine at University of California, San Francisco, who was not involved in the study

Quitting smoking may help improve these factors