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A 2021 risk assessment found that typical diacetyl exposure levels from e-cigarette use could result in hazard quotients up to 200300 times above safe limits, strongly suggesting a significant risk for irreversible small-airway fibrosis (bronchiolitis obliterans) among teen and adult vapers of diacetyl-containing flavors (11)

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At the end of this incubation period, the cultures were centrifuged 10 min at 13,000 rpm, the supernatants were discarded, and each pellet was suspended in 0.6 ml of lysis buffer (1 M glycerol, 0.1 M EDTA)

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