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We determined the copy number by using the following formula: and the following link was used to calculate the final copy number: Generation and analysis of the bulk-RNA-seq data Total RNA was isolated from cells using RNeasy Plus Mini kit (Qiagen) according to manufacturers instructions

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The manager at the Plaza Hotel in Manhattan refused to serve women who wore Le Smoking, while in another noted incident, French singer-songwriter Franoise Hardy was heckled when she wore the Le Smoking to the opera in Paris