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Eluted protein samples were filtered and injected into an autosampler-equipped KTA pure system on a Superdex S75 Increase 10/300 GL column at room temperature using SEC running buffer (20 mM Tris-HCl and 100 mM NaCl (pH 8))

Alcohol Clin Exp Res.2021;45:103950

Nicotinic receptor involvement in antinociception induced by exposure to cigarette smoke

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