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There are a few things that can make your B12 injection less effective or shorten the length of time you can enjoy the benefits

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To prevent this, use the smallest gauge needle practical for drawing doses (29-31 gauge insulin needles), always insert the needle straight (not at an angle), and try to puncture the same spot on the stopper each time rather than creating new holes across the surface

The pancreas then releases enzymes and B12 then clings to a protein called intrinsic factor