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The BALF samples underwent centrifugation at 1,000 rpm at 4C for 10 min ( 2.6 Determination of liver function markers The serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were assessed using commercial kits purchased from Biodiagnostic, Cairo, Egypt as described by 2.7 Liver and lung histopathology After fixation of the liver and lung sections in 10% formalin, they were embedded in paraffin blocks after dehydration with graded ethanol series, cleared with xylene, and then sectioned into 46 m thickness slices

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