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AGS cells were treated with 400 g/mL kanamycin (Thermo Fisher Scientific) in DMEM for 1 h and washed once with DPBS

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ZhouS.2006Clinical pharmacogenomics of thiopurine S-methyltransferase.11(January 2006),119128,1574-8847

In an alkali-burn rat model with companion HUVEC work, Huang and colleagues reported that BPC-157 upregulated VEGF-A expression in wounded skin and accelerated tube formation in vitro, attributing effects to ERK1/2 signaling a pathway-level overlap but not an exact replication of the VEGFR2-internalization finding (Huang et al., 2015)

The animals were then perfused with cold saline to free them of residual blood, after which liver and other tissues were collected immediately, weighed, quickly diced, and frozen in liquid nitrogen at 80 C