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Maybe I should let it macerate for some time

Samples were amplified on a CFX-96 Bio-Rad real time PCR machine using SsoAdvanced universal probes supermix (1725284, Bio-Rad) and TaqMan primers for PARP-1 and GAPDH as housekeeping gene (Hs00242302_m1 resp

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Take, for instance, a 2015 review of the literature on weight and sugar from the World Health Organization, which found that adults and children who consume less added sugar tend to weigh less, and that more sugar in the diet is associated with weight gain