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The membrane was blocked with 5% skim milk for 1 h at room temperature and incubated with primary antibodies Bax, Bcl-2, Keap1, Nrf2, ZO-1, E-cadherin (1:1000 dilution) overnight at 4 , followed by HRP-conjugated secondary antibodies for additional 1 h at 37

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Both of us agreed that a Chief that does his job properly on the train can be an invaluable asset to the crew in ensuring a satisfactory journey for the passengers

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Also, data collectors were instructed to read the survey questions and response options out loud for persons who had questions or needed additional information