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The major pathway of NIC metabolism (in humans) is the oxidation of carbon to cotinine (COT) by CYP2A6 and to a lesser extent by CYP2B6, CYP2D6, CYP2E1, and by glucuronidation, catalyzed by UDP-glucuronosyl transferases (UGT), or oxidation by flavin monooxygenases (FMO