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It was difficult to not have that cigarette holder. Family-friendly Disney banned depictions of smoking on-screen in 2007, after the previous films

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LIF was examined in apical washes from the HBE cultures and mouse BALF using Luminex beads assays (Bio-Rad Magnetic Bio-Plex pro-human Cytokine Bead Panel and the Bio-Plex pro mouse cytokine panel) with the BioRad Bio-Plex 200 system (BioRad, Hercules, CA)