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In order to determine heavy metals, after preparing the samples, first, using metal salts, standard solutions of 1000 mg/L were prepared

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In accordance with these results, upregulation of filaggrin was also observed in oral keratinocytes of smokers 19 , in keratinocyte cell cultures 19, 20 or in reconstructed human epidermis 21 exposed to cigarette smoke extract, and porcine skin explants exposed to cigarette smoke (our unpublished data)

...it is primarily the toxins and carcinogens in tobacco smoke not the nicotine that cause illness and death

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