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Tumorsphere assays Primary tumorsphere formation was assessed in cells grown under anchorage-independent conditions in methylcellulose

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The compounds were identified and quantified using reversed-phase LCMS/MS detection over a theoretical concentration range of 0.200 ng/mL to 100.000 ng/mL

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This is consistent with the incentive-sensitization theory of addiction 4, 5 , which proposes that neutral stimuli can promote drug-seeking behaviour through association with drug effects, and that this is mediated through sensitization of particular brain systems, principally the ventral striatum