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Smoking increases the risk of postoperative recurrence in those requiring resection for CD, likely attributable to perturbations of the gut microbiota although further implications of these for disease onset/progression and treatment efficacy remain unclear

Gas chromatography-mass spectrometry (GC-MS) with a Clarus 600/600 T system (Perkin Elmer, Waltham, MA, USA) was used to quantitatively measure urine cotinine

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