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Because of its widespread use as a food additive and other industrial uses, PG was subjected to standard in vivo toxicological assays many years ago, and these studies, coupled with the relative lack of human evidence of toxicity of PG from its use as a food additive, form the basis for FDAs listing of PG as GRAS

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Western blot analysis The protein was electrophorized in gel and transferred to the membrane (Millipore, Billerica, IPVH85R, MA, USA)

Relationship between flow-mediated vasodilatation and cardiovascular risk factors in a large community-based study