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Phosphofructokinase-1 (PFK-1) PFK-1 is the primary site of regulation of glycolysis PFK-1 is the primary site of regulation of glycolysis,is the primary site of regulation of glycolysis,is the primary site of regulation of glycolysis, is the primary site of regulation of glycolysis, the flux of Fru-6-P to Fru-1,6-BP and, indirectly through the phosphoglucose isomerase reaction, the level of Glc-6-P and inhibition of hexokinase