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Injecting it carries risks of contaminated vials, painful nodules, and systemic copper toxicity

The old culture medium was removed, and the cells were incubated with different concentrations of PDA@Pt for another 24 h

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The volume needed is the same regardless of source, but quality affects whether the peptide stays in solution

For example, lung cancer cell-lines show different sensitivities to doxorubicin in the form of GSH depletion, with A549 and GLC 4 210(S) cells experiencing approximately 50% and 64% GSH depletion after a 12 hour exposure to 70 nM and 5 nM (per million cells) respectively 37