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Discard a vial if any of the signs below show up, or if it has passed its time limit

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This decision was made based on the benefits provided by the assay kit (ability to measure total GSH, free GSH, and GSSG within a single well) compared to other commercially available kits, and our prior positive results with this kit [15]

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Double-blind, randomised study of the effects of influenza vaccination on the specific antibody response and clinical course of patients with chronic fatigue syndrome