Olive oil polyphenols blocked aberrant IL-6/IL-8 expression induced by oxidized sterols through MAPKNF-B signaling, thereby preserving intestinal homeostasis (Serra et al., 2018)
Proteins were transferred to PVDF membranes (Thermofisher) using a Power BlotterSemi-dry Transfer System (Thermofisher) and incubated overnight with IFN- (E3V1X) Rabbit mAb (Cell Signaling, #98139S), Galectin-1/LGALS1 (D608T) Rabbit mAb (Cell Signaling, #12936S, Granzyme B (E5V2L) Rabbit mAb (Cell signaling, #44153) and SHMT2 (E7F4Q) Rabbit mAb (Cell signaling, #3344), vinculin Mouse mAb (Sigma-aldrich, #V9131), actin (clone C4) Mouse mAb (MP biomedicals, #691001) antibodies diluted in 5% skimmed milk in Tris-Buffered NaCl Solution with Tween 20 (TBST)
Los niveles de glutatin en el organismo disminuyen de forma natural con la edad, pero tambin pueden reducirse por factores como el estrs, la malnutricin y las toxinas ambientales
Perilipin ablation results in a lean mouse with aberrant adipocyte lipolysis, enhanced leptin production, and resistance to diet-induced obesity
Rich in antioxidants to help protect against free radicals
