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2.3 Immunofluorescence Analysis (IFA) Four groups of five animals were created: D0 (without mating with males), D1, D3 and D5 (corresponding day of pregnancy)

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(10 g or 10 ng/kg/day), topical (1.0 g in cream), or per-oral (0.16 g/mL in drinking water) over 90 days and described directional improvements across biomechanical, functional, macroscopic, and histological endpoints, though specific effect sizes and p-values are not reported in the abstract

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