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After the cells reached ~ 80% confluence under normal culture conditions, the medium was changed to glucose-free DMEM (Gibco) and the dish was placed in an anaerobic chamber (5% CO2, 95% N2) to induce OGD-related cell injury

They are the concentrations most researchers choose precisely because the math works out to whole numbers or easy fractions on an insulin syringe

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A clean swimmer named Hunter Armstrong told me that his decision to forgo P.E.D.s was about celebrating the talent given to him by God

doi: 10.3389/fmicb.2019.02000 Received 05 May 2019 Accepted 15 August 2019 Published 30 August 2019 Volume 10 - 2019 Edited by Henrietta Venter, University of South Australia, Australia Reviewed by Csar de la Fuente, Massachusetts Institute of Technology, United States