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The GSTs appear to supplement the isomerase activity of the dual function dehydrogenases

and the change in the C-reactive protein (CRP) level

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The increased oxidative stress seems to be the second hit in the progression from NAFL to NASH: the increase in ROS and consequently in lipid peroxidation has been associated to hepatocellular damage, inflammation, and eventual fibrosis due to the activation of hepatic stellate cells

Following standard laboratory practices helps preserve solution integrity and ensures consistent results across experiments