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Statistics and reproducibility Statistical significance was determined using independent t -test for immunohistochemical and western blot analyses, and one-way analysis of variance for cell cycle and apoptosis detection by GraphPad Prism 7.0 software (GraphPad, Inc., La Jolla, CA, USA)

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Insulin-like growth factor-I stimulates both cell growth and lipogenesis during differentiation of human mesenchymal stem cells into adipocytes