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Simultaneously, mitochondria release Smac/Diablo, HtrA2, and Arts, which promote apoptosis by inhibiting the X-linked inhibitor of apoptosis protein (XIAP) (Tower, 2015)

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Uric acid, fetal bovine serum (FBS), penicillin-streptomycin, HEPES, elastase, collagenase, cytochrome c, superoxide dismutase (SOD) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (Merck KGaA)

The supernatants were transferred to HPLC vials and 10 L of the final sample was injected into the chromatographic column

This metabolite can accumulate to high levels when -ECS is overexpressed alone, either in the cytosol or the chloroplast (Noctor et al., 1996, 1998a