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Ferroptosis suppressor protein (FSP-1) and coenzyme Q10 (CoQ10) treatment could attenuate the progression of OA in GPX4-deficient mice [111]

Aging 94 , 130139 (2020)

sgRNA constructs and gene expressing codon optimized (OPT) plasmid constructs sgRNA control and constructs against CBS and L2HGDH were designed targeting GTATTACTGATATTGGTGGG (sgControl) (#230080, Addgene), GGAGCAGACAACCTACGAGG (sg CBS -1) (#230081, Addgene), TGGCAGTGCTGGCAGCACGG (sg CBS -2) (#230082, Addgene), GATATAGTCATCGTTGGTGG (sg L2HGDH -1) (#230083, Addgene), and CACCAGACTGGACATAACAG (sg L2HGDH -2) (#230084, Addgene) and constructed in lentiCRISPR v2-Blast (#83480, Addgene) for Blasticidin selection at a final concentration of 5 g/ml

Given the importance of DNA repair as a mechanism that regulates ANM, we tested whether the same pathways also contributed to PCOS

Finally, the third goal is the reclamation of industrial sites and the development of carbon capture storage capabilities