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Using sterile water without a preservative is an invitation for contamination, which can completely invalidate your research findings

Systemic reactions may include gastrointestinal disturbances (nausea, diarrhea, abdominal cramps) when large doses are administered intravenously

10.1038/s41598-021-01002-1 Sci Rep

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This formation of new blood vessels promotes collagen synthesis, which is crucial for repairing tendons and ligaments, and helps reduce inflammation