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Fluorescence was measured at Ex/Em = 560/590 nm using a plate reader, and LDH release was calculated according to a modified formula to that provided in the kit protocol, expressed as a percentage of LDH release normalized to residual LDH in culture medium and maximum controls

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Therefore, efforts have been undertaken to develop methods to block the P-selectin function [132]

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Hamidi S, Vodopivec DM, Busaidy NL, Gule-Monroe M, Akhave NS, Banuchi VE, Ferraroto R, Fournier I, Gunn GB, Iyer PC, Lee A, Maniakas A, Marczyk VR, Ning MS, Sousa LG, Spiotto MT, Waguespack SG, Wang JR, Zafereo ME , Cabanillas ME, Dadu R